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Each frozen tissue was weighed and dissolved in 200 L of lysing tissue solution (40 mg of EDTA in 1 mL of HClO4 and brought the volume to 100 mL using Milli-Q water) and then bead grinded for 2 min

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181 Shilajit Shilajit, a natural compound found in the Himalayas that is made from microbial decomposition of plants, 182 has been used in traditional Ayurvedic medicine as an aphrodisiac and a treatment for male infertility

doi: 10.1007/s43152-021-00030-3 90 KoakowskiADziemitkoSChmieleckaAywnoHBzdgaWCharytoniukTet al

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Factors like age, physical activity, and climate affect how much fluid each person requires