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These two dimensions of PAIS must be taken into account, in order to have a chance of deciphering interactions between them

The column was pre-equilibrated with 10 CV of DPBS (Sigma-Aldrich)/0.05% (v/v) sodium azide (Sigma-Aldrich), before the supernatant adjusted to pH 7.2 was loaded onto the column

however, because of the higher presence of intracellular proteins (cytoskeletal proteins in the T cell and CAR-T cell cocultures and mitochondrial proteins in the TCR-T cell cocultures), we applied a less stringent cutoff (10% instead of 5% FDR) (Supplementary Fig

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