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Formation of gamma-glutamycyst(e)ine in vivo is catalyzed by gamma-glutamyl transpeptidase

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Density barrier solution preparation 2b-1 Nycodenz To make up the density barrier solution place 50 ml of Solution A, which contains 0.26 M NaCl, 6 mM KCl, 0.6 mM CaNa2EDTA, 10 mM Tricine-HCl, pH 7.2