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Lyophilization helps stabilize peptides by removing water, which can improve storage stability before mixing
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and Zhong C.J
The primary analytical challenges in semaglutide characterization arise from several structural features, including: Acylated lysine residue Fatty acid side chain Spacer/linker chemistry Multiple formulation-associated variants These modifications create substantial analytical heterogeneity that can complicate LC-MS workflows, chromatographic separation, ionization efficiency, and fragmentation analysis
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