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Mice were monitored for health issues daily, and cages were replaced biweekly

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From these molar concentrations the status of the GSSG/2GSH couple was calculated using the Nernst equation E h c = 255 30 log ([GSH] 2 / [GSSG]) in mV [26], assuming an intracellular pH of 7.25 for RBCs and a temperature in vivo of 37 C [27]

Research tracking peptide degradation kinetics has found that tirzepatide stored at proper refrigeration temperatures loses roughly 1-2% potency per month after the expiration date