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The flow rate for each run started 100% B for 2 min at 0.2 ml/min, followed by a gradient starting at 20% A/80% B changing linearly to 70% A/30% B over the course of 1 min at 0.7 ml/min, followed by 70% A/30% B for 5 min at 0.7 ml/min, and finally to 100% B over a course of 5 min

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mild side effects (nausea, skin bumps, epigastric pain) in ~9% of the glutathione group