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The qRT-PCR conditions were as follows: initial denaturation at 95 C for 5 min, denaturation at 95 C for 10 s, annealing/extension at 60 C for 30 s, for 40 cycles

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Furthermore, under combined treatment, 85% of activated neurons in the AP and 50% of activated neurons in the NTS expressed Calcrl

For each case profile, we randomly sampled 1 patient visit from the subset of test set IV visits that match the profile, while ensuring diversity and representation across secondary criteria covering (a) drug classes in active prescription, (b) number of comorbidities and (c) types of comorbidities

We maintained g1437 at a dose of 5 nmol/kg which resulted in significantly greater BW loss compared to all other groups (Fig

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