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North Bergen: Basic Health Publications

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RT-PCR was done using a standard SYBR-green PCR kit (SYBR Premix EX TaqTM II, Takara), and the gene-specific PCR amplification was conducted using the Applied Biosystems StepOnePlus Real-Time PCR System (Applied Biosystems, USA)

Conclusions The antifibrotic effects of GLP-1R activation on pulmonary fibrosis could be attributed to the inhibition of the interaction between NLRP3 inflammasome and PFKFB3-driven glycolysis, and histone lactylation in lung fibroblasts