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~4045 mg tissue was homogenized using a Dounce homogenizer in 1 ml of ice-cold Nuclei Lysis buffer and incubated on ice for 10 min with an additional 2 mL of lysis buffer
coli XL1-blue expressing GSTs A1-1, A2-2, A3-3, M1-1, and M2-2 were grown overnight with shaking in 2TY medium containing 100 g ml 1 ampicillin at 37 C
In a randomized controlled trial of 72 patients with type 2 diabetes and reduced heart rate variability, oral supplementation with 800 mg/day of lipoic acid for four months resulted in significant improvement in two out of four measures of heart rate variability compared to placebo (81)
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