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Effect of neoadjuvant chemotherapy on the immune microenvironment in non-small cell lung carcinomas as determined by multiplex immunofluorescence and image analysis approaches

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Mancias et al., 2014

The remaining fat particles were digested with 0.2% collagenase (Gibco, Billings, MT, USA) in a water bath shaker at 37 C for 30 min, and the mixture was centrifuged at 1000 rpm for 5 min to remove undigested fat

Moreover, enrichment analysis was performed by Metascape (Zhou et al., 2019)