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Micronucleus frequency in human lymphocytes is related to plasma vitamin B12 and homocysteine

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The samples were subsequently reduced with 4 mM DTT for 30 min at room temperature with shaking at 1,000 rpm, followed by alkylation with 10 mM iodoacetamide for 45 min in the dark at room temperature while shaking at 1,000 rpm

Postgrad Med J 73(855):27