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Many in vitro experiments are performed under nonphysiological conditions using high H 2 O 2 concentrations (~ mM)

Particularly, we found several potential hub proteins against NAFLD development with analyses of protein-protein interaction (PPI) network and qPCR assays

Andriole GL, Bostwick DG, Brawley OW, Gomella LG, Marberger M, Montorsi F, et al

In both male and female GHRKO mice, disruption of the GH/IGF-1 axis from birth results in mice that are remarkably long lived, but temporal disruption of this pathway in young adults (aGHRKO mice) improves lifespan only in females 17 , an observation which is consistent with the beneficial effects of targeting IGF-1R signaling post-development on female healthspan and aging in this study

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