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Subsequently, 10 L of CCK8 (APExBIO) solution was added to each well and incubated for 3 h, then the absorbance value (OD) was measured at 450 nm

HPLC helps determine the percentage of the desired peptide present in the final product while identifying potential impurities that could affect experimental outcomes

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Campbell JD, McDonough JE, Zeskind JE, Hackett TL, Pechkovsky DV, Brandsma CA, Suzuki M, Gosselink JV, Liu G, Alekseyev YO, Xiao J, Zhang X, Hayashi S, Cooper JD, Timens W, Postma DS, Knight DA, Lenburg ME, Hogg JC, Spira A

Most clinical research used this route, making it the best-validated approach for DSIP dosing protocols

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