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doi: 10.1016/j.bbamcr.2014.11.006

A physician-supervised protocol that takes muscle preservation seriously addresses it through three non-negotiable pillars: Protein : approximately 1 g per pound of lean body mass daily, maintained consistently even when appetite is significantly suppressed Branched-chain amino acids : leucine in particular directly suppresses catabolism signalling at the source rather than just providing substrate

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Fluorescence signals from samples were then measured on a microplate reader at Ex/Em = 535/587 nm