First, the study population was drawn from a single country, making it difficult to generalize the results to other racial or ethnic backgrounds
Cells were seeded in complete medium consisting of RPMI-1640 supplemented with 10% FCS (Sigma), 1% Penicillin/Streptomycin (GIBCO), 1% L-Glutamine (Sigma), and 55 mM -mercaptoethanol (GIBCO)
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Its ability to simultaneously induce apoptosis, cause cell cycle arrest, inhibit migration, and reduce MMP-2 activity addresses multiple hallmarks of cancer, potentially overcoming the limitations of single-target agents that often develop resistance
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