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Cells were harvested (16,000 x g, 4 C, 2 min) and washed two times with 1 mL washing buffer (50 mM NaCl, 10 mM NH 4 Cl, 10 mM MgCl 2 , 40 mM MOPS, 10 mM IAM, pH 7.3)

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Some people instead take Nacetyl cysteine (NAC), a glutathione precursor, along with B vitamins, selenium, and sulfurrich foods like garlic and onions to boost production naturally