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Other standard reagents used in cell culture experiments included DMEM basal medium, fetal bovine serum, and a penicillin-streptomycin antibiotic mixture, as well as dimethyl sulfoxide (DMSO) and the Cell Counting Kit-8 (CCK-8), which was used to measure cell proliferation

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Peptide APIs can present specific manufacturing challenges, including controlling impurities and aggregation , maintaining product consistency and managing immunogenicity risks