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Parag Mallick, chief scientist at Nautilus Biotechnology, says he thinks the field is understanding now more than ever that tau is not a single protein that goes up and down. For example, in a single-molecule study that Mallick and his team posted to bioRxiv, researchers identified at least 130 different tau proteoforms in brain organoids and donor tissue

Excess DTT and imidazole were removed on a PD-10 desalting column (Merck), and the reduced proteins were eluted with 3.5 mL ice-cold assay buffer containing 100 mM Tris/HCl, 1 mM EDTA, pH 8.0

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Song Z, Uriarte S, Sahoo R, et al

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