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After 3 min of digestion and trapping, peptides were eluted at 0.5 C by a gradient of eluents A (H 2 O + 0.1% (v/v) formic acid) and B (acetonitrile + 0.1% (v/v) formic acid) at 30 L/min flow rate and separated on an ACQUITY UPLC BEH C18 1.7 m 1.0 100 mm column (Waters) as follows: 07 min: 95-65% A

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As a cofactor for enzymatic reactions, it participates in DNA synthesis and repair, part of cellular maintenance