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siRNA reverse transfection and lentivirus infection About 10 nM of siRNA in OptiMEM (250 l) was mixed with 250 l of OptiMEM with 2.5 l of Lipofectamine RNAiMAX (Invitrogen) for 20 min, then with 1.5 ml of cell suspension in regular medium

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For any exposure longer than 30 minutes, or if the pen feels noticeably warm, contact PlexusDx's clinical team before injecting to confirm whether the dose is still viable

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