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Analyses of TIF from tumours with WT Gclc and with Gclc knocked out (KO) showed that intracellular GSH synthesis partially contributed to tGSH levels in the TIF but not serum (Extended Data Fig

Stained membranes were washed three times with TBS-T and stained with HRP-linked secondary antibody donkey-anti-rabbit (Amersham, NAV934) 1:5,000 in BSA 2.5% for 1 h

Shaking damages peptides through mechanical shearing

However, "no known interaction" does not mean "no relationship." Both substances are heavily involved in liver function

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