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Test peptides were administered via either an intravenous or a duodenal catheter (4 rats per group) after overnight fasting, and food was returned following 4 h blood collection

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ATT, Arterial Transit Time

Additionally, non-invasive methods are better suited for repeated or prolonged treatments, which are often necessary for managing chronic conditions (Sawada and Yamakage, 2024)

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When this loop is disrupted at any point, either in terms of timing, duration, or magnitude of feedback impulse signaling, hypothalamic loss of feeding is lost, resulting in obesity