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CRISPR-Cas9-mediated genetic knockdown (KD) of GLP1R To generate CRISPR/Cas9-mediated GLP1R-KD cell lines, single guide RNAs (sgRNAs) targeting GLP1R were designed using the Integrated DNA Technologies (IDT) CRISPR design tool and validated using CHOPCHOP ( (Supplemental Figure S1)

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