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Briefly, bacterial pellets were lysed in buffer (150 mM NaCl 2 , 50 mM Tris, 10% (v/v) glycerol, 1 mM phenylmethylsulfonyl fluoride (PMSF) at pH 7.4 by bead-beating as described for the ATP assay

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Cagrilintide (AM833) Cagrilintide is a long-acting amylin analog designed for once-weekly (OW) SC administration (based on the structure of natural amylin), developed by Novo Nordisk [112]

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The goal is to introduce the water as gently as possible