The primary antibodies used were mouse monoclonal anti-keratin 20 (Abcam, ab854, 1:200), goat polyclonal anti-E-cadherin (R&D Systems, AF748, 1:500), goat polyclonal anti-uroplakin 3a (Santa Cruz, sc-15186, 1:500), mouse monoclonal anti-uroplakin 3a (Fitzgerald, 10R-U103a, 1:50), rabbit polyclonal anti-p63 (GeneTex, GTX102425, 1:1,000), rabbit monoclonal anti-K5 (Abcam, ab150074, 1:100) and mouse monoclonal anti-keratin 14 (Santa Cruz, sc-53253, 1:50)
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The injection process itself is relatively quick and straightforward
Beard JR, Officer A, Carvalho IA, Sadana R, Pot AM, Michel JP, Lloyd-Sherlock P, Epping-Jordan JE, Peeters GM, Mahanani WR, Thiyagarajan JA, Chatterij S (2016) The world report on ageing and health: a policy framework for healthy ageing
Hanrahan AJ, Schultz N, Westfal ML, Sakr RA, Giri DD, Scarperi S, et al
