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The powder was suspended in 2 ml of histone lysis buffer (10 mM Hepes [pH 7.9], 1.5 mM MgCl 2 , 10 mM KCl in Milli-Q H 2 O with the addition of 0.6 mM DTT, sodium butyrate, and 1x cOmplete protease inhibitor EDTA-free immediately before use) and incubated on a roller for 30 min at 4C

The same beneficial effect of KdPT treatment on dextran sodium sulfateinduced colitis was not seen in MC1Rdeficient mice, which suggests a reduced capacity of KdPT to mitigate colitis in the absence of MC1R and a potential role of MC1R in the mechanism of action of KdPT

and even as they account for less than 1% of the intestinal epithelium, they establish a complex signaling network, that is likely to be the first step of integration, involving the information obtained from the gut lumen (63, 88, 89)

ATTENTION Do not exceed the recommended daily dose

Avoid jargon such as phase 2 biomarkers or mechanistic pathways unless context is given

2021, doi: 10.1016/j.xkme.2021.07.007