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To quantitate the magnitude of the calcium signals induced by Hld Se and Hld Sa , we determined the maximum increase in GCaMP6s fluorescence, as well as the area under the curve (AUC) for the GCaMP6s signal upon peptide stimulation

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Cells were then sequentially stimulated first with KRBH buffer containing a low glucose concentration (2.8 mmol/L) for 1 h, followed by KRBH buffer containing a high glucose concentration (16.7 mmol/L) for 1 h

2013;4:e877