SEC was carried out by loading the protein sample to Superdex 200 Increase 10/300GL (GE Healthcare) to obtain the monomer complex
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in contrast, organ/tissue-level techniques like magnetic resonance imaging and computed tomography are required to provide direct estimates of skeletal muscle mass
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GLP-1-bound ECD was shown to be fully extended 11,34 whereas that of compound 2-bound folded down towards the TMD core and penetrated into the orthosteric binding pocket through its N-terminal -helix and loop by inward movement of 9.0 (measured at the C of R40)
