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The primary component is sterile water, but the differentiator is the additive: benzyl alcohol , present at a concentration of 0.9%

IGF-1 induces hypoxia-inducible factor 1-mediated GLUT3 expression through PI3K/Akt/mTOR dependent pathways in PC12 cells

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The next day, samples were incubated with FITC-conjugated, TRITC-conjugated, or Cy5-conjugated (1:1000, Invitrogen, United States) secondary antibody along with 4,6-diamidino-2-phenylindole (DAPI) (1:1000, Sigma-Aldrich, United States) at room temperature for 1 h

The dynamic range of the probe in the cytosol calculated from the respective min and max ratios was about 3-fold (Figure 8C), which is similar to the predictions from the spectral analysis of recombinant roGFP2-iL (Figure 2)