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Amplifications were performed on a CFX384 Real-Time PCR System (Bio-Rad, Hercules, CA)

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In order to determine if the compounds could modulate exogenous LC3 puncta in a manner similar to the change observed in H9c2 cells, we further evaluated the LC3 response using U2OS that were stably transfected with a plasmid for expression of LC3 linked at its N-terminus to GFP

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