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monoclonal mouse GLP-1 antibody [8G9] (Abcam, Cambridge, UK MA) diluted 1:3000, pH 9, polyclonal Rabbit 5HT (serotonin) antibody (Immunostar WI, USA) diluted 1/10,000, monoclonal mouse/human SST ghrelin antibody (R&D Systems #MAB8200) or anti-SST antibody (Dako, #A0566) both used at a dilution 1:500
Together, these results support the hypothesis that cysteine derived from lysosomal cystine efflux limits the activity of the -glutamyl cycle and GSH synthesis
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The non-coenzyme action involves participation of thiamine and/or derivatives, including thiamine triphosphate, in the regulation of ACh synaptic function, consistent with the early data on thiamine as a co-mediator of ACh in neuromuscular synapses, and in allosteric action on metabolic enzymes
