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Cells were washed with PBS, fixed (3.7% formaldehyde/PBS, 20 min, room temperature), washed twice with PBS and permeabilized (0.1% Triton-X100/PBS, 20 min, room temperature)

The microarray data were deposited in the GEO (Gene Expression Omnibus) database with the accession number GSE164941

10.3969/j.issn.1671-3141.2016.105.152(in Chinese)

In addition, inhibiting METTL17 significantly reduces mitochondrial RNA methylation, impairing the translation of mitochondrial protein-coding genes, affecting mitochondrial energy metabolism, and enhancing mitochondrial and intracellular ROS and lipid peroxidation levels

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