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doi: 10.1016/j.lfs.2013.01.001 47 KoehlerJAKainTDruckerDJ

Cells were challenged at 37C in 400 l KHB-BSA and reactions terminated by replacement of buffer with 100 l ice-cold Laemmli sample buffer (62.5 mM Tris-HCl, pH 6.8, 2% w/v SDS, 10% v/v glycerol, 50 mM DTT and 0.1% bromophenol blue)