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Most total B12 assays capture the vitamin with intrinsic factor rather than an antibody, so these assays are generally not vulnerable to heterophile-antibody interference the way antibody-based assays are

The situation changed in the late 1980s with the advent of the so-called soft ionization techniques of electrospray ionization (ESI) (Fenn et al., 1989) and matrix-assisted laser desorption/ionization (MALDI) (Clarke, 2019)

doi: 10.1210/jcem-71-1-79 77 RanceNEYoungWS3rd

J Immunol 1996;157:55975605

Storage & Handling Store refrigerated (28 C), dry and protected from light

Increased Energy Levels : Anecdotal reports suggest that coffee enemas may lead to increased energy and mental clarity