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Raster Image Correlation Spectroscopy (RICS) image capture and analysis INS-1 832/3 SNAP/FLAG-hGLP-1R WT or V229A cells were seeded onto glass bottom MatTek dishes and labelled with SNAP-Surface 647 (S9136S, New England Biolabs) for 15 minutes at 37C in full media, washed and imaged in RMPI-1640 media without phenol red using a 100X oil objective on a Leica Stellaris 8 STED FALCON microscope from the FILM Facility at Imperial College London using confocal settings as previously described (81)

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