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(5)KO+5-HTP group: TPH1 knockout mice were treated i.p with APAP and 5- Hydroxytryptophan (5-HTP, precursor of 5-HT which can reload the 5-HT content, at a dose of 75 mg/kg/day for 3 days by subcutaneous injection before APAP administration) as previously reported 19

These cation-selective ligand-gated ion channels are activated by the excitatory neurotransmitter glutamate and mediate fast excitatory synaptic transmission in mammalian brains 1,2

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