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Finally, increasing solute flux with tight junction disruptors such as calcium chelators is also presumptive evidence of paracellular movement [31]

Furthermore, there is strong evidence suggesting that lipofuscin (a photoreactive substance) is derived, at least in part, from oxidatively damaged photoreceptor outer segments (Drobek-Slowik 2007)

Therefore the overall impact on fertility that ROS has is not a matter of if it is there or not (as mentioned earlier, some oxidative stress is required for various reproductive physiological functions), but when levels of ROS are too high (or antioxidant levels are too low) it becomes pathological to sperm and the ability to get pregnant