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The ELISA fluorescence readings of 355 nm/460 nm (excitation/emission) were carried out using a SPECTRA MAX GEMINI EM microplate reader (Molecular Devices, Sunnyvale, CA, United States) and concentrations were calculated by the SoftMaxPro Software version 7.1 (Molecular Devices, Sunnyvale, CA, United States) using linear curve

For now, Corley said, the benchmark for optimal aging is still diet and exercise.

Acknowledgements We thank all who made contributions to the manuscript

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Consequently, IL-1 can suppress cardiac contractility, promote myocardial hypertrophy, and induce cardiac apoptosis [11, 19]