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Western immunoblot analyses For the preparation of total liver homogenates, frozen liver pieces were homogenized in RIPA buffer (150 mM NaCl, 1% TritonX-100, 0.25% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 1 mM EDTA, 1 mM PMSF, protease inhibitor cocktail, pH 7.4) on ice with a tissue tearor (BioSpec Products, Bartlesville)
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BPC-157 combined with complementary peptides across 8 research categories
Interplay between MTOR and GPX4 signaling modulates autophagy-dependent ferroptotic cancer cell death
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Both the parent and redesigned sensors responded as expected, with the new sensors demonstrating intact functionality, as evidenced by reversible changes in fluorescence corresponding to redox potential shifts ( Figure 1b and Figure S2 )
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