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In a similar manner, bulldog puppies naturally maintain their knees in a flexed position, creating a biomechanical slope in which the anterior cruciate ligament (ACL) is under near-constant load

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They were then embedded in paraffin wax, sectioned into 5 m slices, and stained with hematoxylin and eosin (H&E)

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At the same time, SIRT1 deacetylates multiple transcription factors, such as Nrf2, promotes its entry into the nucleus and activates the expression of antioxidant stress genes, such as HO-1 and GPX4, the activation of which helps mitigate cell damage caused by ferroptosis