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For the untargeted experiment, proteins were digested with 0.7 g of trypsin (sequencing grade, Promega, Madison, WI) and 0.7 g of Lys-C (New England Biolabs) for 1 h at 37 C

Some compounded methylcobalamin products may specify storage at room temperature, refrigerated, or frozen prior to mixing

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doi:10.1152/ajpendo.00122.2002

When to Seek Professional Guidance Consult a healthcare professional if: You suspect B12 deficiency You experience neuropathy symptoms in the feet You want to explore B12 nerve-targeted injection programmes B12 injections in the foot and ankle are most effective when part of a comprehensive neuropathy care plan , including nutrition, lifestyle, and medical support