The hCTR cells were cultured in growth medium consisting of Dulbeccos Modified Eagles Medium (DMEM, Gibco, #31966-021) supplemented with 10% v/v foetal bovine serum (FBS, Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122), 0.6 mg/mL Geneticin G418 (Gibco, #10131-027) and 0.2 mg/mL Hygromycin (Invitrogen, #10687010)
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The supplementary NAD+ dose is 100200 mg IM, two or three times weekly
Unlike CJC-1295, it lacks the modifications that provide DPP-IV resistance
They tell you to "add bacteriostatic water" without explaining why the amount matters, how it changes your syringe readings, or what happens when you pick the wrong concentration for your dosing schedule