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This mechanism becomes clinically important when using high-dose oral supplements (typically 1000 micrograms or more), as it can provide adequate absorption even when the intrinsic factor pathway is impaired

TB-500 is a synthetic version of a naturally occurring peptide involved in cellular repair and regeneration

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Then 3 mL culture medium made of Dulbeccos modified Eagles medium with 10% FBS, 100 U/mL penicillin, and 100 mg/mL streptomycin was added to each well and maintained at 37 C in a humidified atmosphere of 95% air and 5% CO 2