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It is a powerful antioxidant and is involved in many of the body's functions at the cellular level

10.1016/j.cpm.2017.08.010 18 BrufaniM.CeccacciF.FilocamoL.GarofaloB.JoudiouxR.BellaA

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Briefly, 500 ng of RNA was mixed with 5 l of oligo-dT (0.05 g/l) at 70 C for 5 min and then 15 l of master mix (1x ImProm II buffer, 0.05 mM dNTP, 20 U RNasin Ribonuclease inhibitor, 2.5 mM MgCl 2 and 1 U ImProm II) was added