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In vitro studies have documented 40-70% reductions in pro-inflammatory cytokine production, including TNF-alpha, IL-1beta, and IL-6, when inflammatory cells are pre-treated with GHK-Cu (1-5 M) prior to activation with lipopolysaccharide or other inflammatory stimuli

Lipid peroxidation assay Lipid peroxidation staining assay was performed using BODIPY 581/591 C11 dye (Thermofisher, MA) as previously described 19

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This process mimics the natural tanning response that occurs when the skin is exposed to UV radiation but without the need for extensive sun exposure

Pharmacology, 77(2), 78-84