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Mojsov moved to Massachusetts General Hospital in 1983, where she ran the lab for peptide synthesis

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[21] After the solution is deposited into muscle, cholecalciferol diffuses into the circulation bound to vitamin-D-binding protein and undergoes 25-hydroxylation in hepatocytes, yielding 25-hydroxyvitamin D, the principal circulating biomarker of status

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Briefly, cardiac tissues were homogenized in 250 mM HEN buffer (250 mM Hepes, 1 mM EDTA, 0.1 mM neocuproine and 100 M deferoxamine) and centrifuged at 13,000 g for 30 min at 4 C