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This approach not only uncovers intercellular interactions but also provides detailed insights into the cellular microenvironment, offering a powerful framework for exploring complex biological processes and disease mechanisms [287]

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10 min) and incubated with Hoechst 33342 at room temperature for 10 min (10 M, Beyotime, Beijing, China) for nucleus staining

In HepG2 and Hep3B cells, melatonin raised the Bax/Bcl-2 ratio, activated caspase-3, and induced apoptosis through both intrinsic and extrinsic pathways