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The prepared slides were baked, followed by dewaxing, rehydration, staining (employing HE, Safranin O, and immunofluorescence for HIF-1, IL-6, IL-1, TNF-, NF-B(P65)), dehydration, clearing, and mounting

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doi: 10.1128/MCB.01215-12

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second, SCFAs enhance insulin signaling in adipose tissue and liver via FFAR2/FFAR3 (GPR43/GPR41) receptor activation, and their deficiency directly exacerbates insulin resistance