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In particular, the SURMOUNT-1 trial demonstrated that patients on tirzepatide experienced up to 22.5% weight loss , while semaglutide users typically averaged around 15% at the highest dose

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PUF proteins were fused to the LexA DNA binding domain as follows: cDNA sequences encoding PUF repeats of FBF-1 (amino acids 121-614), FBF-2 (121-632), PUF-3 (88-502), PUF-9 (162-703) and PUF-11 (91-505) were each cloned into the Nde I site of pBTM116 using the Gibson assembly method (GIBSON et al