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The docking results of GSH with mTYR indicate that GSH can form extensive interactions with the residues surrounding the pocket

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The supernatant was supplemented with 5 mM imidazole and incubated with 1 ml of Ni-NTA agarose (Qiagen, Cat#30210) for 1 h on a rotary mixer

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Unreconstituted vials should be refrigerated or frozen for long-term storage